FastQCFastQC Report
Mon 20 Mar 2023
SRR6155080_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155080_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133030951
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG5554400.0141.168431
CTGGCAT7616600.0140.585891
GTCGTAG5760150.0140.566941
GATAGAC5189850.0140.260961
CTAACGA4345150.0140.197862
GACTAGT4200050.0140.040821
TATCAGC4747700.0139.987441
ACTTCGA5717450.0139.822512
TCGTAGA4229100.0139.790762
TGGCATA6213450.0139.69872
CACTTCG7457000.0139.634311
GCGAGTA4855550.0139.099321
ACTAGTA3456500.0138.772872
GAGCTGA4068900.0138.575641
ATCAGCA3935600.0138.418372
CGCATAC3378100.0138.294251
CCGTCTA3663500.0137.589862
CTCAATG3036200.0137.576351
ATAGACA4356600.0137.24812
CCGAAGT3312200.0136.761381
Produced by FastQC (version 0.11.5)