FastQCFastQC Report
Mon 20 Mar 2023
SRR6155079_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155079_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117832473
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA4109400.0140.688081
GATAGAC4499650.0140.299871
TATCAGC3978900.0140.230291
TAGCGAC3089650.0139.485582
ATCAGCA3027250.0137.280782
GTCGTAG2976900.0137.213581
TCACGCA3221750.0136.555222
ATAGACA3463550.0136.35522
TTCACGC4414550.0135.130251
ATCATTC3932450.0134.73341
GCCACAT2764350.0134.287931
AGTTAGC2022000.0134.285962
TCGTAGA1956250.0133.974462
TAGGATG4233950.0132.611051
CCTAATC2389600.0132.054141
ATAGACG1111150.0130.962132
AGCCATG1504550.0130.890461
GGCTAAC1856900.0130.852342
CCGTCTA2547950.0129.852722
GAGTTAG2780500.0129.496951
Produced by FastQC (version 0.11.5)