FastQCFastQC Report
Mon 20 Mar 2023
SRR6155079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117832473
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37835223.2109332034472367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG12989041.1023311035829657No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8887000.754206355322781No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA4067420.3451866787180135No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2819600.23928887582627584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC2727290.23145487237631007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2402250.20386994678453368No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2353030.19969283000620722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1934260.16415339089081155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1932770.16402694017972447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1901480.16137147524689566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1601910.135948093018467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1571770.13339022427204766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT59418000.0104.426181
AGCAGTG60734200.0102.1965562
GTATCAA60686000.0102.0722359
AGTGGTA60701950.0102.0471955
GGTATCA60943550.0101.680938
GTGGTAT60938000.0101.6711656
TGGTATC61111050.0101.4249657
CAGTGGT61305000.0101.1498954
GCAGTGG61630550.0100.70663
TAGCGAC80150.030.8252142
CATGGGG14711750.025.65606325-29
ATGGGAG6606000.023.8461225-29
ATGGGGG5690400.023.11848825-29
ATGGGGA4822850.022.9875525-29
ATGGGGC3464450.022.92292425-29
ATGGGGT2870900.022.56570225-29
ATGGGAT6478900.021.7122225-29
ATGGGAC3749400.021.48612825-29
CATGGGT5967400.021.04707525-29
AGAGTAC60230400.020.91534415-19
Produced by FastQC (version 0.11.5)