FastQCFastQC Report
Mon 20 Mar 2023
SRR6155078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110646178
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1755785015.868464973096494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG25626252.3160537908503263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8934260.807462142976145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5630600.5088833705579962No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC4665220.4216340848212579No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4267280.38566899256113485No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA3837290.34680727968750985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA3349580.3027289383642334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2441460.2206547071151432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG2250720.2034159733922305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1952730.176484180050033No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1884970.17036015469056692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1646300.14878959488325028No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1604180.1449828660145857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1506270.13613393858032766No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1485140.13422424767351657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1442740.13039221291493683No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1313550.11871625606444354No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1247970.11278925513360254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC1203730.10879092452700896No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1136430.10270847312954634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTACATGGGAAGCAGTGG1116690.10092440789052833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT130943000.058.881031
AGTGGTA134380950.057.5846335
AGCAGTG134303450.057.4863662
GTATCAA134633000.057.454039
GGTATCA136534750.056.6832438
CAGTGGT136672650.056.6716274
GCAGTGG137123400.056.357843
GTGGTAT137467400.056.3049166
TGGTATC137572800.056.283127
ATGGGGC2738350.020.97477725-29
CATGGGG13644200.020.06418425-29
CCGTAAG852100.019.89333530-34
CGTAAGT841650.019.87001630-34
TGACCGA514500.019.84362870-74
GCCGTAA890650.018.97247330-34
ATGGGGG5105450.018.5756925-29
GAAACCG559500.018.48154385-89
ATGGGAT4994200.018.35747725-29
AAACCGC562250.018.30151285-89
TGGGAAC2003600.018.22202525-29
Produced by FastQC (version 0.11.5)