FastQCFastQC Report
Mon 20 Mar 2023
SRR6155077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132628945
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG78951275.952793336326395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG22615001.7051330688033446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA10369240.7818233041060533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA5907660.44542765532817896No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5651710.42612945462244306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA5437950.4100123091531792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC3625320.2733430474019076No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3318190.25018596053825204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2479640.18696069700320697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2038000.1536617817475665No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1969480.1484954886733058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGAAAAAAAAAAAAAAAA1925610.14518776425462782No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT1454200.10964424093096721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1422870.10728201147947004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1343460.10129463067055235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT91622850.090.487941
AGTGGTA94562650.087.8238455
AGCAGTG94455800.087.8216552
GTATCAA94551900.087.766029
GGTATCA95168350.087.245798
CAGTGGT95593250.086.995574
GTGGTAT95449350.086.991746
TGGTATC95600750.086.879147
GCAGTGG96235000.086.218213
CATGGGG19075000.024.83470525-29
ATGGGGG7577500.023.5331425-29
ATGGGGC3895500.023.12851725-29
ATGGGAG8213000.023.0774625-29
ATGGGGT3553850.022.53396825-29
ATGGGAT7821800.021.94558525-29
ATGGGAC4449700.021.64847225-29
ATGGGGA6385500.021.48062125-29
CGCATAT295900.019.81109635-39
CATGGGT8464300.019.70765125-29
TGGGAAC2267250.019.57583625-29
Produced by FastQC (version 0.11.5)