FastQCFastQC Report
Mon 20 Mar 2023
SRR6155076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115316269
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG112007729.713089139226314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG26216092.273407753072552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA6256250.5425296928397848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6096000.5286331280801324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC5664540.491217765638949No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA4635960.40202133143936525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA3199020.27741272135677575No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2818680.2444303847534297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2579690.2237056420894089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2138750.1854681926970773No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1694820.14697145638660924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT1598940.1386569313996796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1541490.1336749804140819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1332490.1155509115543792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1269150.1100581913554626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1248320.10825185473178985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1169520.10141847374545217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT99184050.073.782911
AGCAGTG101501650.072.1324842
AGTGGTA101787450.072.0257645
GTATCAA102088650.071.791019
CAGTGGT102967750.071.280054
GGTATCA103162300.071.084128
GCAGTGG103283250.070.913343
GTGGTAT103661000.070.742956
TGGTATC103758800.070.693357
CATGGGG14622700.022.80332425-29
ATGGGGC2943150.021.63914125-29
ATGGGAT6145900.021.14004725-29
CGCATAT311700.020.71020935-39
ATGGGGT3043850.020.60960825-29
ATGGGGG6085550.020.37861625-29
ATGTCGC312750.019.9084530-34
TGGGAAC2322550.019.71671925-29
ATGGGGA4699800.019.56812525-29
TCGCATA334350.019.19525335-39
ATGGGAG7582450.019.0711825-29
Produced by FastQC (version 0.11.5)