FastQCFastQC Report
Mon 20 Mar 2023
SRR6155075_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155075_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100607535
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC3718800.0140.800741
GTCGTAG3895800.0140.127031
ATAGCGA2470200.0139.820511
GGCTAAC5239150.0139.67572
TAGCGAC2481450.0139.55922
GTGCGAA4162750.0139.16192
TATCAGC2222400.0138.788561
AGGCTAA5233650.0138.637331
ATAGACA3529700.0138.346082
TCGTAGA3529700.0138.211472
GGTGCGA4633750.0138.032751
AGTTAGC1731450.0136.578322
ATCAGCA2103050.0136.30872
GCGAGTA1836600.0136.225651
TAGGATG3020300.0134.792471
TCACGCA1830750.0134.361022
CGAGTAA1705100.0134.27262
ATGAATC2388050.0134.077332
TGCGAAT1402950.0133.899293
GCTAACT1588750.0133.510673
Produced by FastQC (version 0.11.5)