FastQCFastQC Report
Mon 20 Mar 2023
SRR6155075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100607535
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1015756610.096227881937471No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG15892131.5796162782439707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA7451570.7406572479884335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4856310.48269843804442675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3355150.33348893798063933No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC3203480.31841352638249215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2928760.29110742053266686No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2662120.26460443544313056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2372340.23580142381979638No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1802420.17915357930198766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA1597840.15881911826981945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1523050.15138528143046143No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1369400.13611306548758997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1336790.1328717575676613No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1200850.11935984715260144No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1156820.1149834353858287No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATCTCATCCCCGCCTTGT1047850.1041522386966344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT90972550.074.170721
AGTGGTA93413000.072.261085
AGCAGTG93454400.072.2281952
GTATCAA94205450.071.633419
CAGTGGT94653200.071.3873144
GCAGTGG95115400.071.003293
GGTATCA95097700.071.000228
GTGGTAT95185600.070.921526
TGGTATC95231550.070.919997
CATGGGG13788100.022.70460125-29
ATGGGGG4297900.021.92885825-29
ATGGGGC2672050.021.91602125-29
ATGGGGT3170050.021.33704425-29
ATGGGAG6528600.020.39786125-29
ATGGGAT6632300.020.08631125-29
ATGGGAC3664400.019.921925-29
TGGGAAC1812900.019.17053625-29
ATGGGGA5335800.018.87961225-29
GAAACCG514900.018.81271285-89
TGGGATT2570450.018.40272925-29
Produced by FastQC (version 0.11.5)