FastQCFastQC Report
Mon 20 Mar 2023
SRR6155074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134912655
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG130662449.684965431893694No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG32958112.4429220520491572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA5828450.43201655174601666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5755180.4265856305325842No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC5203720.385710295301801No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA3824840.2835049091577065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG3515510.2605767412997691No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA3337960.2474163746907212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3284420.24344788114947408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG3024950.22421543775860017No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2548160.1888747945846889No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA2196240.1627897694252626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT1929870.14304588402029447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1834150.13595092321028002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC1608500.11922528690877814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1602820.11880427377253823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1556490.11537020007500408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT118203450.076.180021
AGCAGTG120742050.074.610612
AGTGGTA120727050.074.6031345
GTATCAA121661550.074.001189
CAGTGGT122010700.073.882094
GCAGTGG122383850.073.643183
GGTATCA122901850.073.2809758
GTGGTAT122976750.073.2251366
TGGTATC123110650.073.1695257
TGACTGG863750.022.7685182
ATGGGGC3934250.022.32629825-29
GACTGGA878600.022.2607463
CATGGGG18223700.022.224825-29
CGCATAT358900.021.35149435-39
ATGGGAC3907500.020.907125-29
ATGGGGG6628650.020.54850625-29
ATGGGGT4090950.020.16878525-29
GAAACCG849150.019.92718785-89
TACGCAC252750.019.82741250-54
AAACCGC846600.019.81201685-89
Produced by FastQC (version 0.11.5)