FastQCFastQC Report
Mon 20 Mar 2023
SRR6155072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96050735
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1507750015.697433236715991No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13071581.360903693240869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG11854711.2342133560976913No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3449500.3591331185544806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3196510.3327939135499588No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2973260.3095509888602102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1862950.19395478858126386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC1806940.18812349535898917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA1534410.15974994881611265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1479400.1540227672385849No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1468280.15286504574899923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1396940.14543772101275437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA1367270.14234872851311342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1300640.13541176962362653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1288390.13413640197547683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1247780.12990842808230463No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1160600.12083197489326865No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1044900.10878625759605066No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT990500.10312258412181853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT977720.10179203730195298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT114120000.056.1255841
AGTGGTA116181050.055.166295
AGCAGTG116742100.054.887212
GTATCAA116979000.054.7693989
CAGTGGT117825700.054.4623534
GCAGTGG118390350.054.1601683
GGTATCA118502950.054.0827458
GTGGTAT119090200.053.808896
TGGTATC119324850.053.72837
GACTGGA613350.028.8730753
TGACTGG622050.028.6776542
GTGACTG793550.022.5166931
ACTGGAG787600.022.4756854
CTGGAGT791250.022.4656565
GAAACCG727000.021.0583985-89
TGGAGTT855200.020.8530716
ATGGGGC2320700.020.8051225-29
AAACCGC731850.020.73782285-89
CATGGGG11013650.020.71275725-29
AGTTCAG854350.020.5620299
Produced by FastQC (version 0.11.5)