FastQCFastQC Report
Mon 20 Mar 2023
SRR6155071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96334222
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1136548811.797975593761478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG15762351.6362150098643033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA5223180.5421936142277664No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4983630.5173270616126427No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2475240.25694295844315845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2065910.21445234695516616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA1981030.2056413555714396No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1968590.2043500180029481No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC1880510.1952068497527286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1823320.18927022631687418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1708960.17739905555058097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA1665180.17285446079587377TruSeq Adapter, Index 2 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1580670.16408187736233545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA1277050.13256452104839753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1192690.1238075084054761No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1162600.1206840078077342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1116560.11590481314106632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1058570.10988514548858869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGTCGCCAGGAGGAG1045500.10852841059950638No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATCTCATCCCCGCCTTGT1005780.10440526524416215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC991790.1029530295059631No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC978280.10155062029773801No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA971560.10085304887810274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT92468450.068.635351
AGCAGTG94786700.066.9814152
AGTGGTA94829600.066.961655
GTATCAA95670600.066.418139
CAGTGGT95989800.066.21284
GCAGTGG96269800.066.0023353
GGTATCA96578400.065.817688
GTGGTAT96715350.065.722816
TGGTATC96874300.065.632667
GACTGGA597400.025.7270153
TGACTGG621100.024.768512
AACCGAG177900.022.478481
CATGGGG12952150.021.50482425-29
ATGGGGC2845550.021.21925525-29
CTGGAGT740800.020.7591535
ATGGGGG4617950.020.48160725-29
ACTGGAG756600.020.389564
GAAACCG572200.019.8676285-89
ACCGAGA201950.019.5789182
ATGGGGT2796000.019.34579825-29
Produced by FastQC (version 0.11.5)