FastQCFastQC Report
Sun 19 Mar 2023
SRR6155069_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155069_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137893046
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3058070.2217711544351555No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2611060.189354001216276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA5200300.0141.451981
TAGCGAC5077850.0141.071462
TATCAGC3890500.0140.163421
GGCTAAC4853850.0138.971682
GATAGAC3029800.0138.60141
ATCAGCA3626200.0137.92092
AGTTAGC2656900.0137.577182
GTCGTAG2567400.0137.405331
AGGCTAA4998750.0137.391361
ACCTTAC5266250.0136.862782
TCACGCA3233500.0136.822742
TCGTAGA2368300.0135.203032
ATGAATC3460700.0134.874832
GCTAACT1600050.0134.749573
CGACAAC7344950.0134.303712
CCGACAA7561150.0133.930621
AGCGACT1608450.0133.557893
ATAGACA2967300.0133.362962
TTCACGC3582300.0133.337511
GCGAGTA1981050.0133.291731
Produced by FastQC (version 0.11.5)