FastQCFastQC Report
Sun 19 Mar 2023
SRR6155067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120753622
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG67631495.600783552480107No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG38449783.1841512795367746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7849860.6500724259848703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5201760.43077465618381205No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC4663110.386167298567657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3987470.33021535370591204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3155210.2612931974827223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC2946760.24403077532531486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2700250.22361648083732014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2601630.2154494380301073No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2132040.1765611635235256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2017010.16703515526846888No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1998790.1655262978364326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATCTCATCCCCGCCTTGT1957440.16210196991026904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1807510.1496857791975797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1741040.1441811824079281No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTGGGTAAAAAAAAAAAA1703930.14110798266572905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1598970.1324159038475881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC1467530.1215309301446875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTAGGAGCCTCTCTCCCTA1359300.11256805199598899No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1321060.10940127328023337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1319790.10929610045154586No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA1266210.10485896646644687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGTGACAAAAAAAAAAAAA1235580.10232239659030683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATACAATCCATTATATAAGT1231390.10197540906888905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT81919950.096.467591
AGCAGTG84044600.094.3140262
AGTGGTA84339350.094.05615
GTATCAA84501350.093.859349
CAGTGGT84744300.093.6164
GGTATCA84853100.093.486298
GTGGTAT84959500.093.364556
TGGTATC85096250.093.235837
GCAGTGG85145350.093.1425553
GACTGGA809300.026.635923
TGACTGG823550.026.2800162
AGTTCAG807200.026.1696859
CATGGGG18865850.025.05332225-29
ATGGGGG6843750.024.5084725-29
ATGGGGC3902200.023.34931425-29
ATGGGGT4069100.023.13156925-29
ATGGGAG6873600.022.8128325-29
ATGGGAC3625100.022.40680525-29
GAAACCG1280300.021.92314785-89
ATGGGGA5964900.021.86560425-29
Produced by FastQC (version 0.11.5)