FastQCFastQC Report
Sun 19 Mar 2023
SRR6155065_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155065_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119339105
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC3412600.0137.67681
TAGCGAC2349300.0137.625172
TATCAGC2879200.0137.253971
ATAGCGA2505250.0136.607931
GCCACAT4090050.0136.023961
GTGCGAA3854200.0135.430132
CCGTCTA3838050.0135.126112
GGTGCGA4490200.0135.056261
ATCAGCA2581150.0133.94252
GCGAGTA2455650.0133.150381
TCACGCA2365050.0132.781482
CAGATTC2936950.0132.654592
ACCTTAC3614400.0132.342062
AGTTAGC1836850.0131.955692
ATAGACA3196050.0131.253232
TGGAACA7916750.0131.108021
CGAGTAA2217250.0131.072922
CGTCTAG1305600.0130.883333
GGCTAAC1929250.0130.784382
GGCTTCA3472650.0130.710922
Produced by FastQC (version 0.11.5)