FastQCFastQC Report
Sun 19 Mar 2023
SRR6155065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119339105
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21765451.8238321797368933No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG11373530.9530430113415046No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7285160.6104587427566177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6435470.5392591137666065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC3737090.3131488207490747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC3424770.2869780194848956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3326680.27875858462320463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA3164920.26520393294385775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2244520.18807917153392428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGTCGCCAGGAGGAG2070460.1734938434472087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1987980.16658244587974746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1777810.14897128648652092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1657200.1388647920562166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1469370.12312560916222726No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1301990.10910003053902576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT50192500.0115.933441
GTATCAA51081550.0113.895719
GTGGTAT51088700.0113.853986
GGTATCA51121050.0113.825728
AGCAGTG51197250.0113.695462
TGGTATC51276650.0113.479487
CAGTGGT51371850.0113.283874
AGTGGTA51584450.0112.778175
GCAGTGG51862200.0112.2157753
CATGGGG15626100.026.20474425-29
GACTGGA911650.026.1091023
GTGACTG940700.025.532311
TGACTGG955350.025.1561152
AGTTCAG983450.023.9541519
ATGGGGC3961550.023.78616125-29
CAACGCA50790450.023.39064410-14
ATCAACG50973050.023.31151410-14
AGAGTAC50778950.023.28897715-19
CAGAGTA50847600.023.26806815-19
ATGGGGA5090750.023.23833825-29
Produced by FastQC (version 0.11.5)