FastQCFastQC Report
Sun 19 Mar 2023
SRR6155064_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155064_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110295571
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC3114350.0137.974081
GACTAGT3454900.0136.442171
CGACTGG4841600.0136.262241
ACGTGAT2591500.0136.170042
TACCGTC2005800.0135.978353
CCGTGAG3442300.0135.676791
CTACCGT2328550.0135.659122
ACGCTTA2237350.0135.389542
CTCAATG2494100.0135.360531
ATGCCTA1865750.0135.359971
AACATCG3412800.0135.345062
GAGCTGA2857750.0135.270071
GTCACTA2166350.0135.226622
AGTCACT2488350.0135.035481
CGAACTT3439300.0134.718351
ACTAGTA3101250.0134.280872
AGATCGC2304900.0134.005721
CGTGAGA2860850.0133.992312
AACGTGA2622400.0133.233611
ACTTCGA1349000.0131.79122
Produced by FastQC (version 0.11.5)