FastQCFastQC Report
Sun 19 Mar 2023
SRR6155063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114659929
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG113829209.927548446327748No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG43264643.7733007840952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC6317270.5509570828358005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA6250770.5451573234447058No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4035570.3519599249010524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA3530430.3079044292797356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3329270.2903603751577415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG2790870.24340412769660794No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2445120.21324973958426227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2434180.21229561375360698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA2258070.19693628102630342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATCTCATCCCCGCCTTGT2128240.18561323197749408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1887760.16463990658846475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1635820.14266710386677459No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1563680.13637545510777352No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTGGGTAAAAAAAAAAAA1541930.13447854132196435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC1458200.12717607735480108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1236290.10782232387393158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC1235230.10772987658138179No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1227870.10708797839914938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGTGACAAAAAAAAAAAAA1185280.10337351595604075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1182470.1031284434163569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT99455250.077.361381
AGCAGTG101898100.075.4965742
AGTGGTA101905500.075.468615
GTATCAA102518800.075.031449
GTGGTAT103109000.074.562996
TGGTATC103142100.074.5546957
CAGTGGT103184000.074.5446244
GGTATCA103245150.074.494658
GCAGTGG103833800.074.066533
CATGGGG15829450.023.18479525-29
GACTGGA644800.022.7817483
ATGGGGC3257600.022.7369225-29
ATGGGGG5623050.022.38811125-29
ATGGGGT3769900.021.28692625-29
TGACTGG703400.021.0476842
GAAACCG835950.020.87306285-89
CGTAAGT902350.020.5616930-34
AGTTCAG727700.020.1637469
AAACCGC866950.020.0552785-89
ACCGTAC509350.020.026950-54
Produced by FastQC (version 0.11.5)