FastQCFastQC Report
Sun 19 Mar 2023
SRR6155061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108212639
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55036895.085994622125424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG22370512.0672733062170305No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7730980.7144248649180435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC3997910.36944945035487026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3451830.31898584415818565No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3265960.30180947717207046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2367940.21882286781676213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1826060.16874738633811526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1760280.1626686139684663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1595280.1474208571884103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1465920.13546661587284642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1415120.13077215499753223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1394350.1288527858561882No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1292730.11946201589261675No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1178360.10889301017785917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1130430.10446376785987077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT61783750.089.344641
AGCAGTG63187950.087.4665452
AGTGGTA63255750.087.330245
GTATCAA63502200.087.0202269
CAGTGGT63658150.086.808064
GTGGTAT63669250.086.7199866
GGTATCA63755650.086.658818
TGGTATC63803550.086.563077
GCAGTGG63980450.086.3357243
CATGGGG12149250.024.50798425-29
GACTGGA805700.023.1686823
AGTTCAG810700.022.7704739
TGACTGG836750.022.4811462
ATGGGGC2836650.022.3539825-29
ATGGGAG5644650.022.06488625-29
ATGGGGG4965150.021.51678725-29
ATGGGAC2828100.020.99742925-29
ATGGGGT2524350.020.7887925-29
ATGGGGA4041350.020.47028425-29
GAAACCG823550.020.34180585-89
Produced by FastQC (version 0.11.5)