FastQCFastQC Report
Sun 19 Mar 2023
SRR6155060_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155060_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104624436
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCAT2766700.0139.009751
ACGTGAT2154400.0138.562062
GCCAAGT5850900.0138.252522
ATGCCTA1729000.0138.091581
ACGTATC1791700.0137.626881
CTCAATG1741050.0137.098621
TGGCATA2272950.0136.92882
AACGTGA2164100.0136.895081
GTCACTA1803750.0136.760672
GACTAGT1932650.0136.440551
CCAAGTC5427350.0135.378653
CGAACTT1737850.0134.993881
CTATGCA1105050.0134.65952
ACTTCGA1190200.0134.625292
ACGCTTA1556800.0134.513412
CAAGTCC4626950.0134.388874
ACTAGTA1673000.0133.458832
AGTGGTC1870300.0133.448231
TGCCTAA1599250.0132.89672
AAGTCCC5060600.0132.727555
Produced by FastQC (version 0.11.5)