FastQCFastQC Report
Sun 19 Mar 2023
SRR6155059_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155059_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120945222
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC5870000.0141.661422
ATAGCGA6593650.0141.541121
GGCTAAC5568700.0139.444952
TATCAGC2622400.0137.974011
AGGCTAA6318950.0137.347891
GATAGAC2366100.0136.838651
AGTTAGC2666800.0136.646212
AGCGACT1946550.0136.233383
GTCTGTC3289850.0135.264021
GCTAACT1931000.0134.757873
TCACGCA2486700.0134.252782
ATCAGCA2299100.0134.172262
GCGAGTA2290600.0132.875321
TAGGATG4051000.0132.549261
TCTGTCA2840150.0132.333882
TAAGGTC2309700.0132.14762
GTCGTAG1604150.0131.89391
CAGATTC2684300.0131.748352
TCTTCAC5914800.0131.686951
ATCCGTC1408600.0131.329162
Produced by FastQC (version 0.11.5)