FastQCFastQC Report
Sun 19 Mar 2023
SRR6155059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120945222
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43861673.626573193606607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG16296031.3473893164626214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6734000.5567809863543017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5651060.46724127721225733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC4719650.39023038049407194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4084470.33771239015957155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA3868860.31988531138501697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3014560.2492500282483255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2113420.17474191746078238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1760610.14557086017006937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1731590.14317142681337178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1550010.12815801851188466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA1516720.1254055327625923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1354810.11201848056469729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT56107650.092.137491
GTATCAA57280250.090.341899
AGCAGTG57329950.090.246532
GGTATCA57511500.089.950688
GTGGTAT57561150.089.804936
AGTGGTA57578950.089.7988055
TGGTATC57767900.089.515057
CAGTGGT57770450.089.511684
GCAGTGG58194800.088.852733
CATGGGG13618950.025.21681625-29
ATGGGGG5363800.023.3382625-29
ATGGGGC3509050.022.88177125-29
GAAACCG1283750.022.28439985-89
AAACCGC1280500.022.16215985-89
ATGGGAG5419350.021.64533225-29
CGTAAGT847450.021.4553530-34
ATGGGGA4372200.021.16923925-29
CCGTAAG884350.020.78644430-34
ATGGGGT2589650.020.61506825-29
ATGGGAC2537000.020.31523925-29
Produced by FastQC (version 0.11.5)