FastQCFastQC Report
Sun 19 Mar 2023
SRR6155057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117194745
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG63322045.403146702524929No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG19571421.6699912611269387No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4883630.41671066394657885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4824920.4117010536607252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4576080.3904680197051497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2910640.24835925877051912No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2720560.232140101503698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2649100.22604255847819799No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC2513060.21443452946631694No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC2454670.20945222415902692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT1909240.1629117414778282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA1668440.1423647451086651No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1492970.12739223076939157No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1355360.11565023670643254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1188380.10140215757967645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT70430900.088.78131
GTATCAA72275750.086.783219
AGCAGTG72163800.086.722352
AGTGGTA72302250.086.480465
GGTATCA72651400.086.328948
GTGGTAT72829050.086.1359566
CAGTGGT72743900.085.976534
TGGTATC73040150.085.912747
GCAGTGG73173950.085.4733053
GACTGGA817450.026.6318073
TGACTGG868750.025.2747152
CATGGGG14252300.025.1471725-29
AGTTCAG905800.024.182369
ATGGGGC3013750.022.65462125-29
ATGGGGG6078350.022.36821225-29
ATGGGAG6161500.022.1701125-29
ATGGGGT2896650.021.83313425-29
ATGGGGA4621000.021.58404725-29
ACTGGAG1027800.021.39524
CTGGAGT1033250.021.2071365
Produced by FastQC (version 0.11.5)