FastQCFastQC Report
Sun 19 Mar 2023
SRR6155056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116588330
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2296019019.693386121921467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG27194082.3324873081208044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18535531.5898272151252189No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4514160.38718798013489No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA3442030.2952293767309301No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3307010.2836484577830388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC3074040.2636661833993162No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3036690.2604626037614571No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC2483090.2129792921813015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT2408140.2065506899361197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2405210.2062993783340065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2306320.19781739733299208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1946620.16696525286879055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1814750.15565451533614041No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1727790.1481957928379281No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1695080.14539019471331308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1618100.13878747555608695No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1405140.12052149644822942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1398360.11993996311637709No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATCTCATCCCCGCCTTGT1290980.11072977887237943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC1228250.10534930897457748No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1217440.10442211497497218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1182520.10142696100029908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT158951200.056.5221861
AGTGGTA162097950.055.4608655
GTATCAA162653900.055.416529
AGCAGTG162549750.055.3318372
GGTATCA164415500.054.818228
CAGTGGT164027250.054.8173754
GCAGTGG164995600.054.4958153
GTGGTAT165639900.054.438616
TGGTATC165765200.054.4064527
GACTGGA611150.031.7557283
TGACTGG618450.031.3808942
GTGACTG688000.028.1759851
ACTGGAG798600.024.4417324
AGTTCAG793050.024.1159089
CTGGAGT820300.023.8245225
ATGTCGC414050.021.55268730-34
CGCATAT434350.021.51534335-39
TGGAGTT903450.021.4718556
CATGGGG15036150.021.2025625-29
GAAACCG808850.021.1733985-89
Produced by FastQC (version 0.11.5)