FastQCFastQC Report
Sun 19 Mar 2023
SRR6155055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111099472
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG65285705.876328557169021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG17722281.5951722974885068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6126830.5514724678439515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4238440.3814995628422069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3828280.34458129557987455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2438820.2195167948232913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2350840.21159776528910956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2238580.20149330682687674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC2141220.19272998885179218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT1659070.14933194281967424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA1577720.14200967579755913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA1425120.1282742369828724No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1388780.12500329434508925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1297400.11677823275343738No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC1287670.11590244101250095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT65822600.083.5316541
GTATCAA67527900.081.5663459
AGCAGTG67487650.081.486622
AGTGGTA67535450.081.35885
GGTATCA67836600.081.185938
GTGGTAT67983800.081.042026
TGGTATC68186250.080.813137
CAGTGGT68122850.080.684664
GCAGTGG68539300.080.196793
CATGGGG13824750.024.88232625-29
ATGGGGG5602000.023.7963825-29
ATGGGGC3015000.022.10376725-29
GACTGGA795800.021.808623
ATGGGGT2773100.021.57538425-29
TGACTGG819200.021.3087392
GAAACCG957300.021.28043785-89
ATGGGAG5094350.021.05375925-29
AGTTCAG823900.020.9749159
CGCATAT316500.020.90061235-39
ATGGGGA4452300.020.78043425-29
Produced by FastQC (version 0.11.5)