FastQCFastQC Report
Sun 19 Mar 2023
SRR6155054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97583809
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1524463315.62209259529929No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11566991.185339055580419No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG11443411.172675069488218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5226660.5356072952635001No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3263380.33441818201624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2714890.2782111118454087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC1973970.20228458186132087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1884370.19310273080240187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1816040.1861005446098133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1631310.16717015012193262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1545950.15842279737205175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1451500.14874393763416224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1393240.14277368492553924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA1368320.1402199825997774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC1329100.13620087324117466No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1301620.1333848323137294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT1154110.11826859515188633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1142500.11707884860284558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA1055770.10819110371065757No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCAAATTGATCCTGTTTG989280.10137747338802895No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG979210.10034553990406339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT111539850.057.982881
GTATCAA114367700.056.6294639
AGCAGTG114305700.056.6119652
AGTGGTA114277850.056.610195
CAGTGGT115935000.055.8038984
GGTATCA116134250.055.7692958
GCAGTGG116614050.055.471033
GTGGTAT117550150.055.094676
TGGTATC117647950.055.0639277
GACTGGA575250.024.3336523
TGACTGG579150.024.132492
ATGGGGG4762000.021.90112125-29
CATGGGG13020250.021.43944725-29
AGTTCAG656100.021.3007959
GAAACCG685000.020.5926585-89
ATGGGGC2363700.020.27464325-29
AAACCGC690700.020.20380485-89
GCGAACC354400.019.70576950-54
GTGACTG722050.019.4801831
ATGGGGT2801400.019.37520625-29
Produced by FastQC (version 0.11.5)