FastQCFastQC Report
Sun 19 Mar 2023
SRR6155053_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155053_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111566307
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC4244350.0137.915661
ATCCGTC4071750.0135.927872
ATCAGCA3694800.0135.479932
GGCTTCA4784450.0133.677582
AGTTAGC2262550.0133.537722
ATAGCGA1907050.0133.331941
TGGCTTC5625700.0133.263761
TAGCGAC1723400.0133.049442
CTAACGA4307550.0132.922032
GGTGCGA3174400.0132.521471
GTGCGAA2637700.0132.437412
AATCCGT4781000.0131.805421
GTCTGTC2922150.0131.521131
AGGACAC8253300.0131.480132
TGAAGAG6022000.0130.75121
TCTGTCA2507150.0130.625212
GGCTAAC1911650.0130.480822
GCCACAT2249900.0129.954241
CCTAATC1919750.0128.606021
GTCGTAG1505750.0128.072391
Produced by FastQC (version 0.11.5)