FastQCFastQC Report
Sun 19 Mar 2023
SRR6155053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111566307
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG36809143.2993061247424813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG17012231.5248537356354368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6953760.6232849492813274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3299500.2957434093431093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC2641870.23679819392067894No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2293250.20555040869103966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTGACGAAGCGAAATTTG2278820.20425700744939063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC2273320.2037640270731557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2245520.2012722353532774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2214510.19849272235926926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2105290.18870302841520067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1992240.17857004086368117No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC1825070.16358612641001016No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCACGCCTCCTCCAAGTC1656510.1484776223703452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT1593860.1428621277210511No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA1440990.12915996224559087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1322380.11852861635009573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA1321700.11846766604903397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT47805750.097.73161
GTATCAA48881950.095.678329
AGCAGTG49102150.095.254592
GGTATCA49189700.095.103728
AGTGGTA49262000.094.964145
GTGGTAT49324950.094.827046
TGGTATC49537500.094.443417
CAGTGGT49660950.094.1907354
GCAGTGG50076000.093.371943
CATGGGG13858400.025.6690625-29
ATGGGGG5805500.023.7276425-29
ATGGGGA4545800.022.68098825-29
ATGGGGC3144650.022.65636825-29
GACTGGA713650.022.2371673
ATGGGGT2405550.021.65356425-29
CCGGTGA620750.021.597013125-129
GCGAACC702550.021.29163650-54
CGCATAT293800.021.23711635-39
ATGGGAG4113600.020.97534225-29
GAAACCG861400.020.97164585-89
Produced by FastQC (version 0.11.5)