FastQCFastQC Report
Sun 19 Mar 2023
SRR6155052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104638093
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG85753428.19523918502605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG16657821.5919460611729612No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8060490.770320804680567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5339110.5102453463099714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3609210.3449231438114989No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC2439240.23311204648960873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2106210.20128520499699853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA1815880.17353909536558546No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1779420.17005470464757036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT1661390.1587748736972873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1596120.15253718356660037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1563590.14942837308780083No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA1422900.13598298279384735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1416100.13533312385576446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1398680.13366833816438148No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1272180.1215790505662216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC1221420.11672804472841454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC1192630.1139766566655606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA1182350.11299422285916468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT74635900.071.020971
GTATCAA76345350.069.495119
AGCAGTG76606400.069.273592
AGTGGTA77009850.068.9179845
GGTATCA77492650.068.467898
CAGTGGT77910650.068.1041264
GCAGTGG78384450.067.6821753
GTGGTAT78455900.067.621126
TGGTATC78588450.067.5165947
GACTGGA650100.025.1195723
TGACTGG698550.023.4804042
CATGGGG12891350.023.2583925-29
ATGGGGG5129700.022.12438625-29
AGTTCAG736750.022.0077049
ATGGGGC2837500.021.95079225-29
CGCATAT312550.021.64931935-39
ATGTCGC308750.021.00618630-34
GAAACCG894150.020.9523485-89
AAACCGC884400.020.94072285-89
GTGACTG809850.020.1188351
Produced by FastQC (version 0.11.5)