FastQCFastQC Report
Sun 19 Mar 2023
SRR6155049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119643132
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1424205211.903777310008902No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG29434632.4602022287413874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15144231.2657834801583094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11747970.9819176248244654No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6661440.5567757955383514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6115410.5111375720254465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC4617240.3859176805903075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4394250.3672797532582146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT3319080.2774150044818285No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3067130.2563565453970229No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA2685280.22444079782197612No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2394910.20017112223374425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG2366540.19779990380057919No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2351990.19658378719139513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2141970.1790299170703756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1415120.11827841484457294No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1378860.11524773523982973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTGGGTAAAAAAAAAAAA1310850.10956333038824159No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA1280360.10701491833229508No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATCTCATCCCCGCCTTGT1252710.10470387886535769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT130956150.077.0300141
AGCAGTG134857200.075.318642
AGTGGTA134955600.075.224375
GTATCAA135951250.074.697729
CAGTGGT136655600.074.3215944
GGTATCA137044200.074.1234368
GCAGTGG137143450.074.0533753
TGGTATC137333150.073.97597
GTGGTAT137413150.073.924666
TAGCAGT1003200.025.1214221
CATGGGG21004600.023.29647625-29
ATGGGGG6162250.022.85369725-29
ATGGGAT9099550.022.46545825-29
ATGGGGC3494100.022.4637325-29
ATGGGGT5025500.022.2720825-29
CGCATAT610850.021.81240835-39
TCGCATA616050.021.31741535-39
ATGTCGC603600.021.21088630-34
ATGGGAG9015100.021.0698225-29
ATGGGAC5341150.021.06746525-29
Produced by FastQC (version 0.11.5)