FastQCFastQC Report
Sun 19 Mar 2023
SRR6026884_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026884_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205533782
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGG45374950.0142.060031
GACTGGA40485500.0138.98292
ACTGGAT13704550.0138.04193
ATGCCTA4390300.0136.21441
CCACTGT11529100.0134.719712
CGCATAC3853450.0133.487751
ACTGGAA11366500.0132.898733
ACGTATC2441400.0131.572741
TGCCTAA4094600.0131.167222
ACTGGAG14459050.0129.70333
ACGCTTA2183750.0129.555112
CTGGATG4965350.0129.011464
ACTTCGA2065450.0128.259142
AACATCG3515950.0128.182652
ACTGGAC3009450.0128.134743
CTCGGTA2027550.0127.687392
CTGGATA4273350.0126.301414
GTGCGAA3484250.0126.101912
GCTAACG1759200.0125.127821
CTAACGA1556650.0124.146772
Produced by FastQC (version 0.11.5)