FastQCFastQC Report
Sun 19 Mar 2023
SRR6026884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205533782
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG93308564.539816233226322No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23523421.144503826626418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6435180.31309597562896013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG2933920.14274636370968935No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCA2847780.13855532517763916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2803990.13642477517394197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC2295560.11168772245917218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2293990.11161133598952605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT173547800.0109.309311
AGCAGTG177531200.0106.8394852
AGTGGTA177652250.0106.777025
GTATCAA177971300.0106.64329
TGGTATC178208800.0106.4025047
GGTATCA178287250.0106.3933268
CAGTGGT178302600.0106.383564
GTGGTAT178380900.0106.302876
GCAGTGG181251400.0104.629883
CATGGGG28058500.025.2638725-29
ATGGGAT25187200.025.2368825-29
ATGGGAC11361500.024.83696625-29
CATGGGC18799650.024.44399525-29
ATGGGAG23913550.024.25980625-29
ATGGGGG9682800.023.89552125-29
ATGGGCG3034800.023.81457125-29
ATGGGGT6685650.023.52782625-29
CATGGGT11519450.023.24199525-29
ATGGGCT6552700.022.7578725-29
ACATGGG163906050.022.60731120-24
Produced by FastQC (version 0.11.5)