FastQCFastQC Report
Sun 19 Mar 2023
SRR6026883_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026883_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191415812
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCAG19283550.0141.149981
CAGCAGA19248650.0140.705252
CATCAAG5585300.0140.504731
ATGCCTA4296000.0140.27611
CAAGCTA4452200.0140.007572
AGTACAA7358800.0139.836471
AGAGTCA4083500.0139.55991
AGCAGAT6914250.0139.539843
GAGTCAA4097800.0139.050032
CTCGGTA6531600.0138.883652
CTCCTGA13848900.0137.694182
TGCCTAA4379000.0137.687992
ACAAGCT4534050.0137.495991
GATCGCA2756600.0137.295682
ATCAAGT5680250.0137.120422
GCTGATC3757450.0136.705662
GTACAAG7541200.0136.529662
GAACTTA2913200.0136.398472
AGCAGAG6795600.0136.256623
AAGCTAG1755300.0135.849373
Produced by FastQC (version 0.11.5)