FastQCFastQC Report
Sun 19 Mar 2023
SRR6026883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191415812
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG15809280.8259129606283518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4945190.25834804075642404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG2764590.14442850729593856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2639440.13789038493852326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2479700.12954520183525903No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC2455170.12826369850783276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2266170.11838990605436503No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2237810.11690831476346374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2134980.11153624027674369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT135468750.0135.673051
TGGTATC137760100.0133.363227
GGTATCA137931750.0133.240168
GTGGTAT137918700.0133.179386
GTATCAA138170750.0132.994789
AGTGGTA138190650.0132.957355
AGCAGTG138353000.0132.827062
CAGTGGT138511200.0132.673574
GCAGTGG141239150.0130.102223
CAACGCA137455600.027.30585710-14
TCAACGC137632700.027.23656310-14
ATCAACG137557650.027.23112510-14
AACGCAG138276650.027.13703710-14
CGCAGAG137920550.027.12939515-19
AGAGTAC137540000.027.1279915-19
ACATGGG135360450.027.0851220-24
TACATGG137552000.027.07067320-24
CAGAGTA138279550.027.05209215-19
GTACATG137749500.027.04582820-24
GCAGAGT138166350.027.04061315-19
Produced by FastQC (version 0.11.5)