FastQCFastQC Report
Sun 19 Mar 2023
SRR6026882_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026882_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157002741
Sequences flagged as poor quality0
Sequence length150
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8920810.5681945387182762No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2684150.1709619833962007No Hit
CAGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1679270.10695800527457033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGA14895550.0142.799594
CTACGTG23493650.0142.54323
ACGTGAC15227350.0142.474785
TCTACGT28081500.0142.365172
GGCTAAC7502350.0141.832172
CTCTACG28212450.0141.792791
TACGTGC8357700.0141.222444
CGTGACT8979850.0140.771856
TAAGGTC3969650.0140.130922
TGACTGG7129550.0139.92358
GTGACTG7279400.0139.89987
AGGCTAA7579250.0139.751981
ACAGTGC6578350.0139.508822
GACAGTG6448650.0139.355771
ATAGCGA1492300.0139.16031
ATGCCTA2184850.0138.75131
GCTAACT2765550.0138.243123
GAACAGG5091050.0138.133851
GACTGGA5019950.0137.438989
AACAGGC5278700.0136.924472
Produced by FastQC (version 0.11.5)