FastQCFastQC Report
Sun 19 Mar 2023
SRR6026881_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026881_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243021500
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCTA12166550.0140.686072
ACAAGCT12457450.0139.325131
ATGCCTA5086000.0139.309271
AGGCTAA7990950.0138.877081
GGCTAAC8008400.0138.55072
ATAGCGA4560400.0138.46721
TAGCGAC4567700.0137.93522
GATCGCA5882850.0137.724782
CGCATAC5344250.0137.28151
AAGCTAG4823500.0136.969993
GTACGCA3578750.0136.731981
CTCAATG5777900.0136.036641
TGCCTAA5137450.0135.98212
ACTTCGA3204300.0135.904332
TACGCAA3557450.0135.793852
AGATCGC6066550.0135.602951
GCATACA5334150.0135.303442
GTCACTA2960050.0135.060562
GTGGTCA9165100.0134.6672
CAGATTC5446750.0134.361042
Produced by FastQC (version 0.11.5)