FastQCFastQC Report
Sun 19 Mar 2023
SRR6026881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243021500
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG35403301.4567970323613342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10971600.4514662282966733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9326690.3837804474089741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5646460.23234405186372398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG3452510.14206603119477085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2661750.10952734634589945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2543120.10464588524060629No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC2493800.10261643517137373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT150114450.0126.217951
GGTATCA152028800.0124.195028
TGGTATC152124500.0124.037957
AGTGGTA152634050.0123.973475
GTGGTAT152432850.0123.881886
GTATCAA152507500.0123.835519
AGCAGTG153240650.0123.512392
CAGTGGT153502550.0123.22924
GCAGTGG155934500.0121.255813
ACGCAGT861350.037.5115171
ATGGGAT24213300.026.24153125-29
CATGGGG29597450.025.91943425-29
ACATGGG145254450.025.86058820-24
ATGGGAG28182300.025.66195925-29
TACATGG150659200.025.5087120-24
GTACATG151242500.025.47297520-24
AGAGTAC151827200.025.46197315-19
CAACGCA152314550.025.41616410-14
AGTACAT151515550.025.4140820-24
CAGAGTA152284150.025.39326715-19
Produced by FastQC (version 0.11.5)