FastQCFastQC Report
Sun 19 Mar 2023
SRR6026880_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026880_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222537620
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC6025150.0133.794772
GCTGATC5124400.0133.158572
CCTAATC4632700.0131.765871
CTCTATC6191050.0131.109592
CATACCA11300650.0130.494781
AGGCTAA6360100.0130.430731
TCTATCG2308700.0128.456473
CTGTAGC4177700.0128.17241
CTAATCC4413350.0127.879662
ATACCAA10795900.0127.865022
CCTCTAT6604400.0127.6499941
ACGCTTA1925650.0127.249952
CGGATTG4255950.0126.990451
CATCAAG3844050.0126.665261
CAAGCTA4007100.0126.035912
CTGATCG2022750.0125.026963
CCGTGAG3772100.0124.700561
GCTAACT2161650.0124.116563
ACGTATC1476850.0123.841081
GCGAGTA2609050.0123.7367861
Produced by FastQC (version 0.11.5)