FastQCFastQC Report
Sun 19 Mar 2023
SRR6026880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222537620
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG185370588.329853622052756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA40202271.8065381484712564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9220570.4143375848092561No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4297740.19312420075311312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG3833430.1722598632986189No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3782120.16995418572374416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC3188270.14326881001064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2539780.11412811910183995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2391220.10745239389187321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT218590200.089.1275561
AGTGGTA223273050.087.240595
AGCAGTG223599850.087.093722
CAGTGGT224377200.086.808594
GTATCAA224857300.086.792049
GGTATCA225381950.086.513958
GTGGTAT225506600.086.467156
TGGTATC225439550.086.467067
GCAGTGG227586500.085.571583
CATGGGG31147400.024.27647425-29
ATGGGAT19908950.024.26821725-29
ATGGGGG10418550.023.10361125-29
ATGGGAC10078250.023.05652825-29
CATGGGC19326400.022.73666425-29
CATGGGT14275900.022.72217225-29
ATGGGGT6632050.022.68010325-29
ATGGGGC7181200.022.52115225-29
ATGGGAG22855550.022.27981425-29
ATGGGCT6561500.021.90098825-29
ATGGGCG3145700.021.82355525-29
Produced by FastQC (version 0.11.5)