FastQCFastQC Report
Sun 19 Mar 2023
SRR6026879_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026879_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204145115
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG26023550.0135.700091
ACCTTAC7813650.0134.058262
GTCACTA4521750.0133.523352
TATCAGC3893350.0131.276141
CGCTCGA3173250.0130.720672
ACTTCGA2718250.0130.698462
AGTCACT4862550.0130.138151
CACCTTA8107700.0129.876331
CCTTACG2489000.0129.695543
ATCCGTC3118350.0129.668642
ACGCTCG3448900.0129.55481
CCGTGAG4240900.0129.21
GATCGCA3841000.0128.84532
CGAACTT3738700.0127.324231
CACTTCG3008750.0126.407121
TCACGCA3880150.0126.275722
CGCATAC2705900.0125.821281
CGTGAGA4043900.0125.449142
CCGTCTA3658800.0125.063322
ATCAGCA3799250.0124.989482
Produced by FastQC (version 0.11.5)