FastQCFastQC Report
Sun 19 Mar 2023
SRR6026878_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026878_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198904327
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGA9671450.0139.216812
ACTGTGT30229300.0138.210162
CACTGTG33065750.0137.226941
CACTTCG11381750.0136.851971
CTGTGTC17417150.0134.856893
GGCTAAC5993000.0133.576162
AGGCTAA6399900.0132.472351
GTGTCCC13273650.0130.102725
TGTGTCC12279150.0129.946084
CCGAAGT3905200.0129.341031
AGCGTTA3199650.0127.868022
GTGTCAC6858000.0127.7542655
CAGTTCA4698550.0127.65662
GTGTGAC7804900.0127.601945
TATCAGC2971300.0127.234211
CTTCGAG3632200.0127.206063
CTGTGTG15624150.0126.6101763
CCAGTTC5269800.0126.4952851
CTCGGTA3191000.0126.1612
TGTGTCA6514200.0126.043074
Produced by FastQC (version 0.11.5)