FastQCFastQC Report
Sun 19 Mar 2023
SRR6026877_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026877_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187397518
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA9961400.0141.88831
ATCATTC27550350.0141.477921
TAGCGAC10017350.0141.202122
AAGACGG42470700.0139.579421
AGACGGA42105150.0139.138762
TCATTCC28122350.0138.723142
GACGGAT14875350.0138.623023
CATCAAG6265500.0137.758541
CATTCCG9209650.0137.644743
CAGATCT7438900.0136.85121
GACGGAA12569950.0135.829223
ATCAAGT6361450.0135.579712
ACGGATA4354350.0134.494634
CCGTCTA6580150.0133.878362
ACGGATG4858250.0133.231694
AGCGACT3834250.0132.665943
ACGGAAT4572200.0132.153614
ATTCCGA2393150.0131.77964
AGATCTG7756900.0131.295332
CATTCCA8845000.0131.256073
Produced by FastQC (version 0.11.5)