FastQCFastQC Report
Sun 19 Mar 2023
SRR6026877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187397518
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG19593351.0455501337002766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6206740.3312071614523731No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3343330.17840844615668816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3038770.16215636324489632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2782820.14849823144402585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1909930.1019186390718366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1888100.10075373570315908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT134097000.0134.6461
TGGTATC136649050.0131.992897
GTGGTAT136802950.0131.846456
AGTGGTA136904400.0131.778935
GGTATCA136972300.0131.696628
CAGTGGT137162800.0131.553714
AGCAGTG137193900.0131.540562
GTATCAA137305600.0131.436869
GCAGTGG139861200.0128.994983
CAACGCA136502150.026.95275110-14
TCAACGC136675550.026.88764610-14
ATCAACG136652700.026.86992610-14
AGAGTAC136797200.026.8399215-19
AACGCAG137194700.026.80950410-14
ACATGGG132958950.026.79748220-24
CGCAGAG136956500.026.79687515-19
CAGAGTA137287150.026.75658415-19
GCAGAGT137112000.026.75351315-19
TACATGG136725700.026.73522220-24
GTACATG136957000.026.71817420-24
Produced by FastQC (version 0.11.5)