FastQCFastQC Report
Sun 19 Mar 2023
SRR6026876_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026876_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148137730
Sequences flagged as poor quality0
Sequence length150-151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA13354200.0143.5151
TAGCGAC12937900.0142.812562
ATCCGTC8804550.0140.604052
AATCCGT9284350.0139.243291
AGTTAGC5405350.0139.222822
AGCGACT4620900.0138.867163
ACACGAC10159850.0138.424131
CAGATTC6662650.0137.481492
CAGATCT5100800.0136.714891
GGCTTCA7457600.0135.708632
CACGACC9687150.0135.425352
ATGCCTA2347100.0134.38671
ACGACCT3224400.0134.305273
GAGTTAG5888950.0133.765911
GCTGATC2781300.0133.733632
AGCACCT4047300.0133.139021
AGCGACA4178800.0133.075583
GTCTGTC6405650.0133.033331
GTCGTAG3384850.0132.80411
AGATTCG2508750.0132.79173
Produced by FastQC (version 0.11.5)