FastQCFastQC Report
Sun 19 Mar 2023
SRR6026876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148137730
Sequences flagged as poor quality0
Sequence length150-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG63333314.275299074719182No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8822810.5955815577840973No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2547130.1719433664873898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2074330.14002712205729087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT77437400.079.7688451
AGTGGTA78732500.078.879885
GTGGTAT78835650.078.746596
GTATCAA78896000.078.6965269
TGGTATC78564150.078.690687
GGTATCA78639250.078.629698
CAGTGGT79013900.078.495844
AGCAGTG78579300.078.433562
GCAGTGG80702300.076.807113
ACGCAGT1084900.039.3803671
GGTATAA970100.030.19168
TGGTATA1099400.026.732877
CGTGGGA680200.024.930628145
CGCAGTG1835650.023.5460052
ATGGGAT7939550.022.46648225-29
CATGGGG8980400.022.01650625-29
CCGCAAG442400.021.83465145
ATGGGAC3906350.021.22109425-29
ATGGGCG999800.020.72008325-29
CATGGGC6599850.020.62453825-29
Produced by FastQC (version 0.11.5)