FastQCFastQC Report
Sun 19 Mar 2023
SRR6026874_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026874_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221463314
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA8724650.0133.265352
CGACTGG8778950.0133.205811
TATCAGC4692550.0130.65481
ACCTTAC5445200.0129.599992
GAATCTG7334850.0128.697051
GTCGTAG3908850.0127.1019441
GGTGCGA10942750.0126.567421
GACTAGT4102550.0126.318141
TGCGAAT3306900.0126.2985153
AATCTGA6203650.0126.0675052
GTACGCA3868200.0126.0098341
CACCTTA6033100.0124.921431
TCGTAGA3224400.0124.1194152
ATCCGTC2258000.0124.026112
ATCAGCA4236800.0123.902622
TACGCAA3305950.0123.8961262
AGATCGC3617550.0123.7334441
CCTAATC3012300.0122.979411
CCTTACG1887300.0122.734993
ACTAGTA3694250.0121.045592
Produced by FastQC (version 0.11.5)