FastQCFastQC Report
Sun 19 Mar 2023
SRR6026874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221463314
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG137837026.223921132147422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23591171.0652405391170117No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5721200.2583362407373711No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3789980.17113353591376312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2936000.13257274746642686No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2509070.11329506249509118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT151592500.066.440461
AGCAGTG154003550.065.321052
CAGTGGT154237400.065.179354
AGTGGTA153953400.065.125125
GTATCAA154725250.064.931879
TGGTATC154414100.064.7790457
GGTATCA154839900.064.677078
GTGGTAT155004250.064.6737066
GCAGTGG157821350.063.6980553
CATGGGG16662050.022.66228925-29
CATGGGC10697750.020.98775325-29
ATGGGAT10640700.020.881625-29
GTTGTAT1691550.020.7616186
ATGGGGG5331000.020.73103125-29
ATGGGCG1570950.020.44007125-29
TTGTATC1741350.020.1637737
ATGGGAC5420300.019.9758625-29
AAACCGC1522100.019.5465785-89
GGTATAA1437350.019.5046678
ATGGGCT4364550.019.29731625-29
Produced by FastQC (version 0.11.5)