FastQCFastQC Report
Sun 19 Mar 2023
SRR6026873_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026873_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202051976
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3430650.16979047015110607No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2146800.10624988888997552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC17231350.0139.504471
ATAGCGA6211000.0138.260351
TAGCGAC5816000.0138.054932
TCATTCC16079750.0137.299562
CATTCCG5062700.0136.10743
ACGCTTA4409650.0136.078582
AGAGTCA6897600.0134.983111
AGTGGTC9710550.0134.778551
GTGGTCA8897800.0134.434622
GAGTCAA6222700.0133.780582
CGAGTAA4155600.0131.472382
GCGAGTA4781200.0130.944691
CATTCCT6395250.0127.588893
GTCGTAG2541700.0127.04681
CATTGGC3594450.0126.8503652
AACTCAC4834700.0125.6057741
TACCGTC1319650.0125.5250853
CCACTGT6300600.0125.015172
TCGTAGA2216400.0124.038682
TGGTCAG3711250.0123.5277253
Produced by FastQC (version 0.11.5)