FastQCFastQC Report
Sun 19 Mar 2023
SRR6026873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202051976
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG200954659.945690904799665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA59421232.9408883385530467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8737870.4324565477152275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCTGGTTGATCCTGCCAG7100280.351408590035269No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCA6495960.3214994541800472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAACCCATCTCTCCATCACG5560780.2752153238036138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA5486700.27154894045678624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC5158640.25531252413982825No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4431610.2193301984831863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4130260.2044157192503774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA3579540.17715936616229877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2522140.12482629717018952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG2484500.1229634101672928No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG2279390.1128120617835482No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCAAAAAGAAAGGAGTTCAA2173930.10759261270476266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT221491150.078.94311
AGTGGTA226177300.077.315775
AGCAGTG226479400.077.199432
GTATCAA226869000.077.103679
CAGTGGT226872150.077.074214
GTGGTAT227624300.076.788096
TGGTATC227650250.076.7847447
GGTATCA227831550.076.756398
GCAGTGG230593950.075.823483
CATGGGG28860350.024.26067525-29
ATGGGGG10222300.023.74189425-29
ATGGGAT16054500.023.64844725-29
CGATTAC1141050.023.45937565-69
ATGGGAC9441500.023.41018725-29
ATGGGGT5818550.023.21408725-29
GCGATTA1170650.023.1835565-69
ATGGGGC5798200.022.9799225-29
ACACTCG1319650.022.77325230-34
CATGGGC16827050.022.71110225-29
ATTCGGT1043450.022.70772495-99
Produced by FastQC (version 0.11.5)