FastQCFastQC Report
Sun 19 Mar 2023
SRR6026872_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026872_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183877233
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA11518700.0139.003222
GTGCGAA8654500.0138.65122
ATCATTC9382450.0136.871411
TTCACGC11891500.0136.219921
CGTGAGA6474250.0135.948972
TCGTAGA3990750.0135.794282
GTCGTAG4085350.0135.708191
CGAACTT4903300.0135.295671
GAACTTA4891500.0134.516042
TGCGAAT3237400.0134.514593
CCGTGAG6656050.0134.053991
ACTTCGA2179400.0133.88182
CACGCAT4259700.0133.700473
GTACGCA2169350.0132.566121
GGTGCGA9128450.0132.340481
CAAGCTA3260450.0132.332672
GATGTAC2644700.0131.7332
GCTGATC2895100.0131.676182
TCATTCC9787900.0130.88192
TACGCAA2185100.0130.630042
Produced by FastQC (version 0.11.5)