FastQCFastQC Report
Sun 19 Mar 2023
SRR6026872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183877233
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21400421.163842834202318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13180410.7168048912287036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6067930.32999898361533425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA4213340.22913875368137607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3824540.20799421100707993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT3112090.16924825054333942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC2612350.14207033450410905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA2123340.11547595998467085No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1914990.10414503028768113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT120018000.0131.67321
GTGGTAT121970350.0129.543056
GGTATCA122010700.0129.528438
TGGTATC122021600.0129.490077
GTATCAA122161800.0129.301459
AGCAGTG122345350.0129.141722
CAGTGGT122415800.0129.107884
AGTGGTA122492800.0129.035395
GCAGTGG124762050.0126.654073
CAACGCA121286450.026.50342610-14
ACATGGG119372400.026.43213720-24
AGAGTAC121532250.026.42782815-19
ATCAACG121669150.026.42246410-14
TACATGG121508950.026.39217220-24
TCAACGC121931400.026.38495410-14
GTACATG121722850.026.36720520-24
CAGAGTA121865700.026.35858515-19
AGTACAT121856650.026.3434320-24
GCAGAGT121864300.026.33354215-19
CGCAGAG122016000.026.3126515-19
Produced by FastQC (version 0.11.5)